ClinChem: basic spectrophotometer

00:01:54
https://www.youtube.com/watch?v=QjVXz_SuTC0

Zusammenfassung

TLDR本视频详细介绍了光谱光度计的工作原理。光源发出的光经过透镜聚焦后进入一个有单色仪的腔体。单色仪可以是棱镜或衍射光栅，其作用是将光分解成不同颜色和波长的单色光束。通过移动出口狭缝，可以选择合适的波长让光通过样品池。样品池中包含特定浓度的样品，样品会吸收一部分光，剩余的光则被光电探测器接收并转化为电信号，然后输出吸光度值，例如0.31。

Mitbringsel

  • 🔦 光源提供照明
  • 🔍 单色仪分解光线以选择波长
  • 🧪 样品池包含测试样品
  • 💡 光通过样品时会被吸收
  • 🔅 剩余光线被光电探测器检测
  • 📈 输出电信号表示吸光度
  • 💧 吸光度值示例：0.31

Zeitleiste

  • 00:00:00 - 00:01:54

    بۇ سۈرەتتە سەپەك ئېسطىراپ ئۆلچىگۈچنىڭ ئاساسلىرى كۆرسىتىلگەن. ئۇنداقتا، بىز ق نور مەنبەسى بىلەن باشلايمىز، نۇر لىنزا ئارقىلىق ئۆتۈپ، تىرىكچىلىك خانىسىغا كىرىدۇ. بۇ خانىدا بىر مونوكروماتور بار، بۇ رامكا يەكى مۆجىزلەش قىلىش مەشىقلىرى مۇناسىۋىتى بىلەن نۇر تۇراچىلىققا ئايرىلىدۇ. بۈركۈن ئۇ نور بىرىنچى رەڭدىكى نۇر بەلگىلىمەلەرگە ئايرىلىدۇ ۋە ئاخىرقى سۈرەتتە سىز سۇئاللىرىڭىزنى تالىشىڭىز ئۈچۈن قۇلايلىق يارىتىش ئارقىلىق سىز قوشۇمچە رەڭ ئۈچۈن تۈركۈملىرىڭىزنى تاللاپ كۆرۈلۈش قىلىش ئۈچۈن قۇيۇلۇپ تۇرماققا سېغىنىش ۋە سىغىمدىلىق قۇبۇل قىلىدىغان ئىشلارغا قاتناشماسقا تۇلۇق.

Mind Map

Video-Fragen und Antworten

  • 光谱光度计的基本组成部分有哪些？

    光谱光度计包含光源、单色仪、样品池和光电探测器等基本部分。

  • 单色仪的作用是什么？

    单色仪的作用是将光分解为单一颜色的光束，以便进行分析。

  • 光通过样品池后会发生什么？

    光通过样品池后会被部分吸收，其余部分则被传输到光电探测器进行检测。

  • 光电探测器的功能是什么？

    光电探测器的功能是测量传输光的强度，并将其转化为电信号，以显示吸光度。

  • 视频中提到的吸光度值是多少？

    视频中提到的吸光度值是0.31。

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Untertitel
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Automatisches Blättern:
  • 00:00:00
    all right the basics of a spectr
  • 00:00:03
    photometer so first of all you all going
  • 00:00:05
    to have a light source and um the light
  • 00:00:07
    is going to shine through um usually a
  • 00:00:10
    lens or something that condenses the
  • 00:00:12
    light and it will go through an entrance
  • 00:00:14
    slit into this chamber that contains a
  • 00:00:19
    monochromator so this can be a prism
  • 00:00:21
    like represented here or it can be a
  • 00:00:23
    defraction grading of some sort the idea
  • 00:00:26
    of a monochromator is it uh splits the
  • 00:00:29
    light into to Mono one color color so be
  • 00:00:33
    beams of a single color that's
  • 00:00:36
    monochromator so uh this prism will take
  • 00:00:39
    this beam of light and split it into
  • 00:00:42
    different uh colors and different
  • 00:00:44
    wavelengths um of the electromagnetic
  • 00:00:47
    spectrum and and then you have an exit
  • 00:00:50
    slit and this can be moved up and down
  • 00:00:53
    to select whichever wavelength uh that
  • 00:00:56
    is appropriate for the sample being
  • 00:00:58
    analyzed so here we have green
  • 00:01:00
    coming through and so we've selected
  • 00:01:03
    green and so this green part of the
  • 00:01:06
    light is going to come through and hit
  • 00:01:08
    our uh sample here sample cuvette and um
  • 00:01:12
    and then our sample cuvette has sample
  • 00:01:14
    in it at a set
  • 00:01:16
    concentration and so some of this light
  • 00:01:18
    is going to be absorbed by the sample
  • 00:01:20
    and the rest of it going to be
  • 00:01:21
    transmitted on into onto the photo
  • 00:01:24
    detector so the photo detector's job is
  • 00:01:27
    to uh detect the amount of light that's
  • 00:01:29
    been transmitted
  • 00:01:30
    uh through the sample cuetes um and so
  • 00:01:33
    and its job is to convert this
  • 00:01:35
    electromagnetic radiation EMR or light
  • 00:01:38
    this wavelength it uh is going to
  • 00:01:41
    convert it into an electrical signal
  • 00:01:43
    into electrons and that's going to be
  • 00:01:45
    transmitted to a readout and it will
  • 00:01:46
    give you the number of the absorbance
  • 00:01:49
    for the readout so here it would be 0.31
Tags
  • 光谱光度计
  • 单色仪
  • 光源
  • 样品池
  • 光电探测器
  • 吸光度
  • 电信号
  • 光传输
  • 分析技术
  • 电磁辐射