Do It Yourself (DIY) Test for Garlic Bloat Nematode

00:08:58
https://www.youtube.com/watch?v=ven5cLVn-Eo

Ringkasan

TLDRThis video tutorial demonstrates how to conduct a DIY test for garlic bloat nematodes, also called onion eel worms, in garlic. The initial method involves slicing garlic and soaking the samples in chlorine-free water with optional hydrogen peroxide before examining them with a smartphone for any movement, which indicates a positive presence of nematodes. If the results are unclear, a more complex method using a Behrman funnel can be employed to isolate nematodes from garlic samples. Finally, using a microscope can provide clearer visualization of the nematodes and their life stages. The process emphasizes that while detecting nematodes is crucial for seed garlic, they do not pose a risk when consuming the garlic.

Takeaways

  • 🔍 DIY method for testing garlic for nematodes.
  • 🪴 Garlic bloat nematodes are also known as onion eel worms.
  • 📱 Use a smartphone with proper lighting to check samples.
  • 💧 Soak garlic slices in chlorine-free water before examining.
  • 🧪 The Behrman funnel is an alternative, more thorough testing method.
  • 🔬 A microscope provides definitive identification of nematodes.
  • 🌱 Finding nematodes indicates seed garlic is infested.
  • 🍽 Eating garlic with nematodes is safe; concern is for seed use.
  • ⏳ Let samples soak for 10 to 30 minutes for best results.
  • 💡 Proper lighting is essential for successful observation.

Garis waktu

  • 00:00:00 - 00:08:58

    The video describes a DIY method for testing garlic for the presence of garlic bloat nematodes, also known as onion eel worms in the UK. The narrator emphasizes that while many believe a university lab test is necessary, this test can be done effectively at home with a few tools. The process begins with slicing garlic thinly, soaking it in chlorine-free water, and optionally hydrogen peroxide, before examining it under a smartphone camera placed on a makeshift mount. The examples demonstrate how to identify moving nematodes, which indicate an infection, versus non-moving fibers that could be mistaken for them, thus allowing viewers to assess their garlic's health and potential implications for seed usage. Additionally, the narrator suggests that if the initial test does not yield clear results, a more refined test using a Behrman funnel and blending the garlic can be employed. This method facilitates the separation of nematodes from the garlic, making further examination possible. The video ultimately highlights the practical steps individuals can take to monitor for nematode infestations in their garlic without needing lab tests.

Peta Pikiran

Video Tanya Jawab

  • What is garlic bloat nematode?

    It is a pest (also known as onion eel worm) that affects garlic and can cause significant damage.

  • Can I perform the test at home?

    Yes, the video provides a DIY method to test for nematodes without needing a lab.

  • What tools do I need for the test?

    You'll need a razor blade, glass slide, chlorine-free water, hydrogen peroxide, a smartphone, and optionally, a microscope.

  • What indicates a positive test for nematodes?

    Seeing moving worms in the garlic slices indicates a positive test.

  • Is eating garlic affected by nematodes safe?

    Yes, the nematodes do not affect the edibility of the garlic; the concern is primarily for using it as seed.

  • What is the Behrman funnel method?

    It is a way to separate and identify nematodes from plant material using a sieve and blender.

  • What kind of microscope can I use for testing?

    Any common microscope should work to see nematodes clearly.

  • What lighting works best for the tests?

    Internal fluorescent lighting or external flashlight can help illuminate the samples for better visibility.

  • How long should I let the garlic soak?

    Soaking for 10 to 30 minutes is recommended before observing.

  • What should I do if I find nematodes?

    If you find significant numbers, avoid using garlic from that patch as seed.

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Gulir Otomatis:
  • 00:00:00
    this is a do-it-yourself test for the
  • 00:00:04
    presence of garlic bloat nematode or
  • 00:00:06
    stem involve nematode and UK they call
  • 00:00:10
    it Onion eel worm in your seed garlic or
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    in garlic that you gonna use from seed
  • 00:00:17
    from someone else it's a big problem
  • 00:00:19
    right now in the in the US and most
  • 00:00:23
    people say you need a university lab
  • 00:00:25
    test but you can do it at home here's
  • 00:00:27
    out when I get the slices pretty thin I
  • 00:00:31
    use a razor blade put them on a glass
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    slide piece of glass and you need some
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    chlorine phillipe free water I'm
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    assuming that water might kill not sure
  • 00:00:48
    about that just put a couple drops right
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    on them and soak them good with water
  • 00:00:54
    these slices and I've also had pretty
  • 00:01:00
    good luck adding a bit of hydrogen
  • 00:01:02
    peroxide I don't know whether that's
  • 00:01:04
    because it oxygenates the water or
  • 00:01:08
    whether it may be help some break up the
  • 00:01:11
    cells add a couple drops I've tried it
  • 00:01:14
    several different ways and I let it fit
  • 00:01:22
    up for about 10 20 or 30 minutes before
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    I bother to take it to my smartphone to
  • 00:01:28
    get a closer look here's my setup for
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    the smartphone that's a black ceramic
  • 00:01:33
    coffee mug upside down on a flashlight
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    it's about three inches beneath the lens
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    of my smartphone which is just sitting
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    on a shelf there ain't downward and
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    there is a fluorescent light behind
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    everything that kind of lights things up
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    there's gives you a better idea about
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    how far away and needs to be this is an
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    iPhone 5 by the way
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    this is movie footage from the view of
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    the iPhone it's that zoomed in yet of a
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    slide the light in this case is below
  • 00:02:16
    interest use the touch screen to zoom in
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    and then you want to move things around
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    so you're looking at a dark part of the
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    frame and then you basically press on
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    the touch screen hold it down and it
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    locks in that focus and on that exposure
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    and this is what you can see with the
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    garlic slabs still on the slide in
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    person you could make out that one worm
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    that one ill worm swimming and then on
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    the computer you can see there's a few
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    more of them around but they're a little
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    hard to see a lot of them are actually
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    hiding on the margin between the garlic
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    slabs and the glass there they seem to
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    be hugging that spot where there's a
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    extra thick film of water but as long as
  • 00:03:11
    you have the exposure set for the dark
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    part and you're over exposing the garlic
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    slabs then that should call up anything
  • 00:03:18
    if it's swimming then it's a positive
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    test for nematode if it's just a fiber
  • 00:03:25
    it's not moving it could be any number
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    of things on your on your glass it's
  • 00:03:31
    probably should keep looking now I've
  • 00:03:34
    taken the garlic slabs off and we're
  • 00:03:39
    gonna zoom in again here and see what's
  • 00:03:42
    available that seems to really show a
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    lot more of them I think that the
  • 00:03:50
    majority of them were hiding right
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    underneath the sliver of garlic I don't
  • 00:03:56
    know why possibly there's more energy
  • 00:03:58
    there more food to to consume but again
  • 00:04:04
    this is you know you know microscope to
  • 00:04:08
    find these things if they're in your
  • 00:04:09
    garlic and any significant numbers than
  • 00:04:11
    just one slice two slices out of glass
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    slide
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    with the right lighting and a smartphone
  • 00:04:19
    with a zoomable camera you can just find
  • 00:04:21
    them yourself and basically that means
  • 00:04:24
    that the entire patch that the Scarlett
  • 00:04:26
    came out of is gonna be very suspect for
  • 00:04:29
    being invested with garlic blows
  • 00:04:31
    nematode and it's you know gonna cause
  • 00:04:35
    you problems if you use it as seed it's
  • 00:04:37
    not an issue for eating the garlic okay
  • 00:04:41
    now we're sliding over to a follow-up
  • 00:04:43
    test if your first check didn't work out
  • 00:04:46
    too hot for being a slam dunk positive
  • 00:04:49
    it's called a Behrman funnel it's been
  • 00:04:52
    around since like 1917 and this is how
  • 00:04:56
    the university labs would do a test only
  • 00:04:58
    they would have a higher tech equipment
  • 00:05:02
    chopping up the garlic putting it in a
  • 00:05:06
    blender with some distilled or chlorine
  • 00:05:08
    free water and you want to chop it you
  • 00:05:13
    know you don't want to pureed it you
  • 00:05:16
    just want to chop it and I don't really
  • 00:05:17
    know how far this is the key part of it
  • 00:05:19
    here is you got a sieve that sits on top
  • 00:05:23
    of a bowl I'm gonna dump the blender
  • 00:05:27
    contents into the sieve if you need a
  • 00:05:30
    little bit more liquid to add some more
  • 00:05:31
    water
  • 00:05:32
    and so basically it's soaking and then
  • 00:05:35
    there's about an inch of clearance below
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    I've also added a little hydrogen
  • 00:05:39
    peroxide to get a-movin phones up a
  • 00:05:42
    little bit can make a mess it's kind of
  • 00:05:46
    what it might look like and now
  • 00:05:48
    basically you're going to take wait
  • 00:05:51
    about ten twenty thirty minutes an hour
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    two hours you're going in with a plunger
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    of some kind and you're sucking the
  • 00:05:58
    liquid from the bottom of the bowl and
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    then so that's where the worms wind up
  • 00:06:04
    is at the bottom and you prop up your
  • 00:06:06
    suction device so that the thing is the
  • 00:06:10
    openings at the bottom and that helps
  • 00:06:12
    the worm sink or put it on a glass slide
  • 00:06:15
    a drop at a time you can do this over
  • 00:06:17
    and over and over again without a
  • 00:06:19
    microscope basically you're back to the
  • 00:06:21
    same setup here zooming in on the water
  • 00:06:24
    droplets and again the lighting really
  • 00:06:29
    helps
  • 00:06:30
    this is a video and you can see them
  • 00:06:32
    moving there the droplets stand up
  • 00:06:35
    really good in this case I'm not using a
  • 00:06:37
    black ceramic coffee mug for the
  • 00:06:39
    backdrop but more just black
  • 00:06:41
    construction paper I don't really know
  • 00:06:45
    what the magnification is an iPhone 5
  • 00:06:49
    but it's the zoom is not an optical zoom
  • 00:06:52
    it's a just a digital crop zone type
  • 00:06:58
    thing but if you need to move you take
  • 00:07:01
    some movies and then move the movies to
  • 00:07:03
    your computer that'll help you see and
  • 00:07:07
    then I turned out the lights and tried
  • 00:07:08
    it with a flashlight from the other
  • 00:07:10
    direction just to see if that would work
  • 00:07:12
    and if you don't have under counter
  • 00:07:16
    fluorescent lights like I have then this
  • 00:07:18
    would also be a way of lighting up the
  • 00:07:22
    bubbles and seeing what you can see now
  • 00:07:24
    we're sliding over to taking a look at
  • 00:07:28
    these eel worms using a microscope which
  • 00:07:30
    I happen to have borrowed from a brother
  • 00:07:32
    of mine
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    most of the stuff that's out there on
  • 00:07:36
    the Internet the official wisdom is that
  • 00:07:39
    you need a lab test to be sure well if
  • 00:07:41
    you got a microscope it's a slam-dunk
  • 00:07:43
    situation here you won't have any
  • 00:07:44
    trouble seeing these at all this is the
  • 00:07:47
    lowest power setting some hydrogen
  • 00:07:49
    peroxide bubbles a little bit closer up
  • 00:07:55
    this is all these tho movies were shot
  • 00:07:58
    through the eyepiece of the microscope
  • 00:08:01
    these shots here are a ones that I put
  • 00:08:04
    in the freezer and then thought out
  • 00:08:06
    mighta killed them like that it's hard
  • 00:08:09
    to say also you can see some larvae
  • 00:08:14
    their eggs their younger generations of
  • 00:08:16
    the worms there's two different samples
  • 00:08:18
    one in a figure 8 that's actually alive
  • 00:08:20
    and the other like a pill shape that's
  • 00:08:23
    in a younger Marvel stage they're right
  • 00:08:27
    in there right in the garlic with that
  • 00:08:28
    would be older the older juveniles I
  • 00:08:32
    guess so it was fun and
  • 00:08:36
    boy phenomenal just phenomenal how many
  • 00:08:40
    be singer living
Tags
  • garlic bloat nematode
  • DIY test
  • onion eel worm
  • seed garlic
  • microscopy
  • Behrman funnel
  • home methods
  • plant pests
  • infestation
  • agar plating