Zebrafish Blood Testing Demonstration

00:01:52
https://www.youtube.com/watch?v=LcqPYrxkDxg

Sintesi

TLDRThe video provides a detailed procedure for blood collection from fish, beginning with the preparation of heparinized needles and an anesthetic solution. Fish are anesthetized in the solution, after which blood is collected from the dorsal aorta or posterior cardinal vein using an aspirated tube. Blood glucose levels are measured using a glucometer, and proper post-collection care is given to ensure the fish stabilize and recover.

Punti di forza

  • 🧪 Needles prepared with glass capillary.
  • 💧 Anesthetic solution included tricaine.
  • ⏳ Fish soaked in anesthetic for 2 minutes.
  • 🩸 Blood collected from specific sites.
  • 📏 0.3 microliters of blood needed for testing.
  • 🔬 Blood glucose measured using a glucometer.
  • 🕊️ Fish gently returned to water post-collection.
  • 💨 Oxygen supplied until fish recover.

Linea temporale

  • 00:00:00 - 00:01:52

    Needles were prepared with a 1mm outer diameter glass capillary, with tips cut and heparinized using saline. Fish were anesthetized in a solution for two minutes prior to blood collection from the dorsal aorta and posterior Cardinal vein. A glucometer was used to measure blood glucose after collecting a specific volume of blood, followed by care to stop any bleeding and ensuring the fish returned to a clean tank with oxygen supply until recovery.

Mappa mentale

Video Domande e Risposte

  • What type of needle was used for blood collection?

    A 1 millimeter outer diameter glass capillary needle was used.

  • How was the fish anesthetized?

    The fish were placed in an anesthetic solution for two minutes.

  • What equipment was used to measure blood glucose?

    A glucometer, specifically the Albert Freestyle Light, was used.

  • How much blood was collected?

    0.3 microliters of blood was collected.

  • What was done after blood collection?

    Gentle pressure was applied to the puncture site, and the fish were returned to a tank with clean water and oxygen.

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Sottotitoli
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Scorrimento automatico:
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    needles were prepared using a 1
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    millimeter
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    outer diameter glass capillary with a
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    needle puller needle tips were cut and
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    heparinized using heparinized saline and
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    air dried for 1 hour
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    an aesthetic solution was prepared using
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    200 mils system water and 2 mil 0.4%
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    trike a fish were put in an aesthetic
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    solution for two minutes before blood
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    collection
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    an aspirated tube was used to perform
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    blood collections with collection sites
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    at the dorsal aorta and posterior
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    Cardinal vein after 2 minutes fish were
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    anesthetized and removed from solution
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    and placed on a damp cloth for blood
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    collection a glucometer albert freestyle
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    light with blood glucose strips
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    requiring 0.3 microliters of blood were
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    used the needle was inserted into the
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    blood collection site at a third a 45
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    degree angle and using an aspiration
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    tube 0.3 microliters of blood was
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    collected blood was expelled directly
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    onto a blood glucose strip and a blood
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    glucose measurement was obtained gentle
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    pressure was applied to the puncture
  • 00:01:28
    site to stop any bleeding fish were
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    returned to a tank of clean system water
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    and oxygen was supplied wheezing a
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    Pasteur pipette until fish began to
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    breathe and swim normally
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    you
Tag
  • blood collection
  • anesthesia
  • glucometer
  • fish care
  • scientific procedure