Praktikum Farmakologi - Uji Antipiretik

In this video, the process of conducting an antipyretic test using paracetamol is outlined, focusing on the preparation stage for the test. The first group is tasked with testing the effects of paracetamol at a dosage of 100 mg per kilogram of body weight. Initially, the weight of the test animal, a mouse weighing 26 grams, needs to be measured, which is critical for calculating the appropriate dose. The calculated dosage for the mouse is 0.26 ml of paracetamol, which will be administered orally. Before treatment, the normal body temperature of the mouse is checked using a thermometer. Subsequently, an induction with 10% pepton is performed, where the amount given is based on the animal's weight, resulting in a 0.26 ml intraperitoneal injection. After a 15-minute waiting period, the temperature is recorded as 39.8 degrees Celsius, indicating fever before the oral administration of paracetamol. The rectal temperature is monitored again at specific intervals following the administration of the medication, after which the percentage protection will be calculated.