Polymerase Chain Reaction - PCR #DNAAmplification #TaqPolymerase #GeneResearch #MedicalDiagnostics

00:00:46
https://www.youtube.com/watch?v=EguQYM84Ipw

Resumen

TLDRPwosesis polymerizasyon se yon teknik nan biyoloji molekilè ki pèmèt syantis yo fè milyon kopi segment DNA espesifik. Sa a se yon fason rapid ak efikas pou ogmante DNA pou aplikasyon tankou klonaj jèn, analiz forensik ak dyagnostik maladi. Metòd sa a depann sou yon anzim ki reziste a chalè ki rele DNA polymerase pou repwodwi DNA. Pwosesis la gen twa etap prensipal: denaturasyon, kote fil DNA yo chofe pou separe yo; lyen, kote primer kout yo atache sou sekans DNA sib la; ak etensyon, kote DNA polymerase ajoute nükleotid pou konstwi nouvo fil DNA.

Para llevar

  • 🔥 Polymerizasyon se yon teknik enfòmasyon molekilè pou repwodiksyon DNA.
  • 🧬 Metòd la pèmèt jenetik yo fè milyon kopi DNA.
  • 🧪 Aplike nan klonaj jèn, analiz forensik, ak dyagnostik maladi.
  • 🌡️ Itilize yon anzim ki rele DNA polymerase ki reziste a chalè.
  • 🌀 Pwosesis la separe fil DNA yo anvan li repwodwi yo.

Cronología

  • 00:00:00 - 00:00:46

    Reaksyon chenn polymérize se yon teknik ki lajman itilize nan biyoloji molekilè pou fè milyon kopi spesifik segment DNA. Li se yon fason rapid ak efikas pou ogmante ADN pou aplikasyon tankou klonaj jèn, analiz kriminèl, ak dyagnostik maladi. Metòd sa a depann sou yon anzim ki reziste a chalè yo rele DNA polymerase pou replike ADN. Pwosesis la gen twa etap prensipal: denaturasyon, kote yo chofe fil ADN yo pou separe yo; antranjman, kote primer kout yo mare ak sekans ADN vize a; ak ekstansyon, kote DNA polymerase ajoute nucleotide pou konstwi nouvo fil ADN.

Mapa mental

Vídeo de preguntas y respuestas

  • What is polymerize chain reaction?

    It's a technique used to make millions of copies of specific DNA segments.

  • What are the applications of this technique?

    Applications include gene cloning, forensic analysis, and disease diagnosis.

  • What enzyme is used in this process?

    A heat-stable enzyme called DNA polymerase.

  • What are the main steps in polymerize chain reaction?

    The main steps are denaturation, primer binding, and extension.

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Desplazamiento automático:
  • 00:00:01
    polymerize chain reaction is a widely
  • 00:00:03
    used technique in molecular biology that
  • 00:00:05
    allows scientists to make millions of
  • 00:00:07
    copies of specific DNA
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    segment it's a fast and efficient way to
  • 00:00:12
    amplify DNA for applications such as
  • 00:00:14
    Gene coling forensic analysis and
  • 00:00:18
    disease
  • 00:00:19
    diagnosis this method relies on heat
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    stable enzyme called DNA poas to
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    replicate DNA the process involves three
  • 00:00:28
    main steps denaturation
  • 00:00:30
    where the DNA strands are heated to
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    separate
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    them aning we short primers bind to
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    Target DNA
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    sequence and extension where DNA
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    polymerase adds nucleotides to build new
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    strands of DNA
Etiquetas
  • DNA
  • polymerase
  • chain reaction
  • molecular biology
  • gene cloning
  • forensic analysis
  • disease diagnosis
  • denaturation
  • extension
  • nucleotides