Tes Fehling pada Karbohidrat
Résumé
TLDRThe video demonstrates the Fehling test for detecting carbohydrates using glucose, lactose, and starch. The test involves mixing the samples with Fehling's solutions A and B, heating them, and observing color changes. Glucose and lactose react positively, turning red, while starch shows minimal change. Water acts as a control, resulting in no reaction. The test identifies reducing sugars by the reduction of copper ions, forming a red precipitate of copper(I) oxide, showcasing the presence of these sugars in the samples tested.
A retenir
- 🔬 Fehling test detects carbohydrates.
- 🍬 Samples include glucose (mono), lactose (di), and starch (poly).
- 💧 Water serves as a negative control.
- 🧪 Mix samples with Fehling's A & B solutions.
- 🔥 Heating causes color changes indicative of reactions.
- 🔵 Blue color indicates initial state of solution.
- 🟥 Red color of precipitate indicates reducing sugars.
- ⚗️ Glucose and lactose yield a red precipitate; starch does not.
- 🧪 Reaction demonstrates the reducing properties of sugars.
- 🔗 Copper(II) ions reduce to form red copper(I) oxide.
Chronologie
- 00:00:00 - 00:02:43
In this video, the presenter introduces the Fehling test for carbohydrates, utilizing three samples: glucose, lactose, and starch, as well as aquades as a control. The preparation involves mixing Fehling A (copper sulfate) and Fehling B (sodium potassium tartrate) with the samples and a strong base (NaOH). Upon heating, glucose and lactose produce a red precipitate indicating a positive reaction, while starch shows only a slight change, and aquades remains negative. This demonstrates the presence of reducing sugars in glucose and lactose, as they can convert Cu2+ from Fehling's solution to Cu+. The video concludes with a brief summary of the experiment and its significance.
Carte mentale
Vidéo Q&R
What is the purpose of the Fehling test?
The Fehling test is used to detect reducing sugars, such as glucose and lactose, in carbohydrate samples.
What are the samples tested in the video?
The samples tested are glucose, lactose, and starch.
What solutions are used in the Fehling test?
Fehling's solution A (copper sulfate) and solution B (sodium tartrate) are used.
What color indicates a positive reaction in the Fehling test?
A red precipitate indicates a positive reaction, confirming the presence of reducing sugars.
What happens to the color of glucose and lactose during the test?
They change from blue to red after heating.
Does starch give a positive reaction in the Fehling test?
No, starch shows minimal color change and does not give a positive reaction.
What is the role of the water sample in the test?
The water sample serves as a negative control, showing no reaction.
How does the presence of reducing sugars affect the copper ion?
Reducing sugars reduce Cu2+ to Cu+, resulting in the formation of red copper(I) oxide.
What is the characteristic color of the precipitate formed?
The characteristic color of the precipitate is red-orange.
What should you do to observe the reaction clearly?
You should heat the mixtures to observe the color change.
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ULTIMA CENA.mpg
PART 1❗️SOAL PPPK TEKNIS TERBARU JABATAN PENGELOLA LAYANAN OPERASIONAL
- Fehling test
- carbohydrates
- glucose
- lactose
- starch
- reducing sugars
- copper sulfate
- sodium tartrate
- color change
- laboratory experiment