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[BUGS CHIRPING]
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[MUSIC PLAYING]
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SEAN CARROLL (VOICEOVER):
The diversity
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of animals on our
planet is breathtaking.
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Millions of species adapted
to all kinds of habitats.
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Ever since Darwin, understanding
how so many species evolved
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has been a major quest of
biology and biologists,
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like Jonathan Losos.
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In the Caribbean, he's studying
a remarkable group of lizards.
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[MUSIC PLAYING]
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JONATHAN LOSOS:
She will be back.
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SEAN CARROLL (VOICEOVER):
He's finding clues
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to their origins in their
bodies, their lifestyles,
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and in their DNA.
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SEAN CARROLL: There's
one out there.
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SEAN CARROLL (VOICEOVER):
These lizards
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are providing fresh insight
into both how new species form
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and why our world is filled
with so many creatures.
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[MUSIC PLAYING]
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JONATHAN LOSOS: Don't think
I don't see you, lizard.
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SEAN CARROLL (VOICEOVER):
Here in Puerto Rico,
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Jonathan is stalking
lizards called anoles.
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JONATHAN LOSOS: OK, here we go.
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SEAN CARROLL (VOICEOVER): With
almost 30 years of practice,
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he's a pro at catching them.
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JONATHAN LOSOS: Come on.
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There we go.
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He's OK.
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They've got very strong necks.
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This actually doesn't
hurt them at all.
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He's a healthy, fine
looking specimen.
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SEAN CARROLL (VOICEOVER):
Puerto Rico's anoles
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all feed on similar
food, mostly small
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prey like spiders and crickets.
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But they divide up their
habitats in a clever way.
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[MUSIC PLAYING]
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The long tailed,
slender species Jonathan
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caught lives in
grasses and bushes,
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and it's called a
grass-bush anole.
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On the low parts of tree
trunks and on the ground,
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a longer legged,
stockier species forages,
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called a trunk-ground anole.
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And higher up the tree
lives another anole species.
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[MUSIC PLAYING]
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JONATHAN LOSOS: On twigs and
small branches like these,
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we find very small anoles
with really short legs.
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SEAN CARROLL (VOICEOVER):
This slender lizard
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is called a twig anole.
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Further up the tree is
yet another species.
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JONATHAN LOSOS: High
up in the canopy,
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there's a large green
lizard with big toe pads.
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He lives high off the ground.
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There's one right there.
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[MUSIC PLAYING]
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SEAN CARROLL (VOICEOVER):
Like apartment dwellers,
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each species lives in a
different vertical space.
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But here, each floor
offers unique evolutionary
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opportunities to
its inhabitants.
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[MUSIC PLAYING]
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The fact that lizards differ
in leg length and toe pad size,
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depending on where
they live, suggests
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that these differences in traits
are adaptations to the lizards'
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habitats.
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JONATHAN LOSOS: Here's
a good tree over here.
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SEAN CARROLL
(VOICEOVER): To test
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whether that is,
in fact, the case,
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I came here to help Jonathan
conduct some experiments.
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JONATHAN LOSOS: Yeah, these
lizards are very cooperative.
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SEAN CARROLL (VOICEOVER): We
begin by comparing the running
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ability of two lizards--
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SEAN CARROLL: Short legs.
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Yeah.
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SEAN CARROLL (VOICEOVER):
--one with long legs,
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the other with short ones.
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JONATHAN LOSOS:
Let's do some tests.
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Let's start with
this little lizard
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here and see how fast it can
run up this broad surface.
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SEAN CARROLL: All right.
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I'll catch him if he
makes it to the end.
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JONATHAN LOSOS: All right.
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Here we go.
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There he comes.
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SEAN CARROLL: Wow.
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She's a sprinter.
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JONATHAN LOSOS: Exactly.
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She lives at the bottom of
trees right in the open.
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She catches prey on
the ground, so she
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has to run down
quickly to get them.
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SEAN CARROLL (VOICEOVER): The
shorter legged twig lizard
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is not nearly as fast.
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It seems like a disadvantage.
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Why aren't their legs longer?
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Jonathan puts a twig lizard on
a thin branch to demonstrate.
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JONATHAN LOSOS: All right.
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Let's see how he does.
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There we go.
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SEAN CARROLL: Looks
pretty comfortable there.
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JONATHAN LOSOS: Yeah.
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SEAN CARROLL: Just
sort of scurrying along
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like a balance beam.
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JONATHAN LOSOS: This
is what they love.
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SEAN CARROLL (VOICEOVER):
Instead of speed,
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the twig lizard's legs
provide a firm grasp.
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JONATHAN LOSOS: All right.
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Now, let's try the other one.
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SEAN CARROLL: So
this is the sprinter.
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JONATHAN LOSOS:
This is a sprinter.
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Let's see how she fares
on this little stick.
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Look how ungainly she is.
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Her legs are too long for this.
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So you can see on
these narrow surfaces,
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long legs are a disadvantage.
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SEAN CARROLL (VOICEOVER): On
twigs, long legs only increase
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the chance of falling.
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So ground lizards
have evolved long legs
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and twig lizards short ones
that enabled their lifestyles.
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[MUSIC PLAYING]
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Next, we compare how well
two species can climb
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the slick surfaces of leaves.
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Anoles have different sized
toe pads on their feet.
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We'll see if these
help them navigate
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different environments.
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[MUSIC PLAYING]
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JONATHAN LOSOS: So it's time
for lizard Olympics part two.
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SEAN CARROLL: All right.
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I'm game for that.
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JONATHAN LOSOS: Here's
the ground lizard.
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Let's see if he can
hang on and move up it.
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Oh.
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SEAN CARROLL: No.
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JONATHAN LOSOS:
Couldn't even hang on.
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SEAN CARROLL: Cannot hang on.
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JONATHAN LOSOS:
Let's try it again.
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SEAN CARROLL: Here he goes.
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He's getting up there.
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JONATHAN LOSOS: He's able to
move up, but not very easily.
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All right.
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Let's do another species.
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SEAN CARROLL: All right.
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Oh, my goodness.
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JONATHAN LOSOS: Take
a look at this guy.
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SEAN CARROLL: That's an anole?
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JONATHAN LOSOS: This is
the big canopy lizard.
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Let's see how he does.
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SEAN CARROLL: Well,
that's not a fair contest.
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He's huge.
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There's no way for him
to hold up his weight.
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JONATHAN LOSOS: What do
you think now, smart guy?
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SEAN CARROLL: OK,
you proved me wrong.
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Pretty impressive.
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JONATHAN LOSOS: He's using
the little microscopic hairs
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on his toe pads to
bond with the surface,
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and that's what holds him up.
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SEAN CARROLL: And his toe pads
are bigger than other lizards?
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JONATHAN LOSOS: Yes, they are.
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He's a bigger lizard,
but even for his size,
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he has particularly
large toe pads.
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SEAN CARROLL: So this
is an adaptation.
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JONATHAN LOSOS: This is an
adaptation because he cannot
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afford to fall
out of the canopy.
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[MUSIC PLAYING]
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SEAN CARROLL (VOICEOVER): But
how do these adaptations arise?
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Jonathan and his
colleagues wanted
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to see if they could observe
the lizards' traits evolve
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by conducting another
kind of experiment.
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Their inspiration was the
rapidly changing environment
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of some of the smallest
Caribbean islands.
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[MUSIC PLAYING]
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[WIND WHISTLING]
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Hurricanes occasionally
swamp these tiny islands,
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scrubbing them free of lizards.
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The team realized they could
use the depleted islands
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as laboratories.
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They began their experiment
by capturing tree dwelling
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anoles on a larger island.
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SEAN CARROLL: Oh,
there's one out there.
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JONATHAN LOSOS: Yeah.
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[MUSIC PLAYING]
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SEAN CARROLL
(VOICEOVER): Then, they
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visited seven islands
that a hurricane
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had cleared of lizards.
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On each, they placed a
female and male anole.
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These islands have no
trees, only small bushes.
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How would the long legged
lizards fare on thin branches?
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The next year, the
scientists returned.
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JONATHAN LOSOS:
She will be back.
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SEAN CARROLL
(VOICEOVER): They found
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that the mating pairs they had
introduced not only survived,
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but reproduced.
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And the new population
had grown and taken
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to living on thin branches.
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JONATHAN LOSOS: And
now, she's in my noose.
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SEAN CARROLL (VOICEOVER):
The scientists
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collected the lizards.
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JONATHAN LOSOS: Every
time we found a lizard,
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we measured how high
it was off the ground--
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SEAN CARROLL: 40 centimeters.
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JONATHAN LOSOS: --the
diameter of the surface,
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and whether it was perched head
up, head down, or horizontal.
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SEAN CARROLL (VOICEOVER):
They brought them back
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to their field lab, took
x-rays to precisely measure
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the length of their legs,
and scanned their toe pads.
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Then, they returned each
lizard to the exact spot
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where they had found it.
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JONATHAN LOSOS: OK.
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All right.
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SEAN CARROLL
(VOICEOVER): Now, they
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had baseline data on
the new populations.
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A year later, they came back--
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JONATHAN LOSOS: All right.
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I think he gave us a slip.
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Excellent.
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SEAN CARROLL (VOICEOVER):
--and discovered
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that the average lizard
leg had shortened
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in just two generations.
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JONATHAN LOSOS: We
thought maybe this is just
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a fluke, a statistical accident.
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In fact, over four
years, the populations
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all got shorter and
shorter and shorter legs.
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Evolution can occur
very rapidly when
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natural selection is strong.
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SEAN CARROLL
(VOICEOVER): Adaptations
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like these explain how
different body types evolve,
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but they do not explain how
new anole species arise.
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It's changes in
other traits that
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play a key role in speciation.
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Two groups of animals are
defined as different species
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when individuals from one
group don't mate and reproduce
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with those from the other.
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So for a population to
become a new species,
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something has to
prevent its members
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from breeding with members of
closely related populations.
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This is called
reproductive isolation.
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One way a species
can split into two
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is for populations to
separate geographically.
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Over many generations, they
can undergo enough changes
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in their respective
habitats that if and when
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they come back together
again, they don't mate.
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So what kind of changes
keep anoles from mating?
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Anoles have a flap of
skin under their throats
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called a dewlap, which males
display to attract females.
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[MUSIC PLAYING]
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And remarkably, every
species in the same area
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has a different dewlap.
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[MUSIC PLAYING]
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So a change in a dewlap
is a critical step
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in the formation of
new anole species.
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SEAN CARROLL: Jonathan, why
would these dewlap colors
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change?
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JONATHAN LOSOS: Consider
this grass lizard
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that lives here in the forest
where it's relatively dark.
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And if you look
at its dewlap, you
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can see it's pretty
light colored.
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Now, suppose that a
population of these lizards
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ended up in an area that was
much more open and sunnier.
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In that case, a light colored
dewlap isn't very effective.
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So over time, the
population would
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evolve by natural selection
to have darker dewlap,
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and we might end
up with this one.
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He's got a much darker
dewlap, much more visible
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in a light, open habitat.
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SEAN CARROLL (VOICEOVER):
If for some reason
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these two populations
come together,
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the females would no
longer recognize the males
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as members of the same species.
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They wouldn't mate.
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They would be
reproductively isolated.
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SEAN CARROLL: There's a simple
connection between changes
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within populations,
or microevolution,
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and the formation of new
species, or macroevolution.
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When changes within populations
include traits involved
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in mating, like dewlap
color, then the stage
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is set for the formation
of new species.
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SEAN CARROLL (VOICEOVER):
Once new species are formed,
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competition drives the evolution
of different body types.
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Species living in the same
area compete for resources.
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But if members of one species
move into another habitat,
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they can use resources not
available to the other species.
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Over many generations,
natural selection
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favors traits that
enable species
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to occupy different habitats.
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This process has led to the body
types we see in Puerto Rico.
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And not just there.
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On each of the Caribbean's
four largest islands--
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Puerto Rico, Jamaica,
Cuba, and Hispaniola--
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we find the same distribution
of similar looking lizards.
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SEAN CARROLL: Now, you'd
think that all the lizards
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on the different islands would
look different, but they don't.
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Each island has the
same basic body types.
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SEAN CARROLL
(VOICEOVER): Each island
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has slender grass-bush
anoles with long tails,
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long legged trunk-ground
anoles, short legged twig
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anoles, and canopy anoles
with large toe pads.
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How did each island end up
with the same body types?
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Did each body type
evolve once, and then,
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spread to the other islands?
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Or did each type evolve
independently on each island?
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SPEAKER: So I'm going
to be sequencing
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some additional markers--
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SEAN CARROLL
(VOICEOVER): To find out,
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Jonathan and his colleagues
sequence the DNA of anoles
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from each island.
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They examined the same stretch
of DNA from many species
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to uncover their
evolutionary relationships.
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JONATHAN LOSOS: Species that
are more closely related,
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we wouldn't expect to have
many differences in their DNA.
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For example, these
two species here.
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If you go across here,
there's only one base pair
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where they're different.
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That's because they're
very closely related.
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On the other hand, this species
here has many differences,
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here, here, here, and here.
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That's because this species
diverged from the other ones
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a long time ago.
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SEAN CARROLL (VOICEOVER):
After determining
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which two species were
most closely related,
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they joined them together
with a node representing
00:15:16
a common ancestor.
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Then, they joined these to
the next most closely related
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until all the lizards were
united in a phylogenetic tree.
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[MUSIC PLAYING]
00:15:31
The DNA revealed a pattern
consistent with this.
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The lizards on each island
tend to be more closely related
00:15:38
to each other than to
similar looking lizards
00:15:42
on different islands.
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That means that generally
the same types of lizards
00:15:47
evolved independently
on each island.
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SEAN CARROLL: On all of the
large Caribbean islands,
00:15:54
the same traits have
evolved again and again--
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body color, limb
length, toe pad size.
00:16:01
[MUSIC PLAYING]
00:16:06
SEAN CARROLL (VOICEOVER):
Moreover, this
00:16:08
repeated filling of habitats
on each island by anoles
00:16:12
illustrates why our planet
has so many species.
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SEAN CARROLL: The
simple reason why
00:16:18
there are so many
species in the world
00:16:20
is that there are
so many habitats.
00:16:23
SEAN CARROLL (VOICEOVER):
And each habitat
00:16:24
provides numerous
ways to survive.
00:16:28
In the Serengeti, zebras eat
the tallest, coarsest grass.
00:16:34
Wildebeest, the
medium height grass.
00:16:38
And Thomson's
gazelles, the shortest.
00:16:42
In the Galapagos,
some finches primarily
00:16:45
eat seeds on the ground, and
others, insects in the trees.
00:16:53
SEAN CARROLL: Look around
you in your backyard
00:16:55
or around the world.
00:16:56
There are so many
different environments,
00:16:58
each full of creatures making
a living in a different way.
00:17:01
[MUSIC PLAYING]