Extraction of Nematodes from Soil

00:09:19
https://www.youtube.com/watch?v=x_X08MdV5qk

Summary

TLDRThis video provides a step-by-step guide on processing scientific samples using straining and centrifuging techniques. It begins with gently mixing 100 CC's of the sample, placing it into a strainer, and blasting it with water to remove debris. The strainer must be cleaned between samples to avoid contamination. The sample is then poured onto a pre-wet 400 sieve at a 45-degree angle and rinsed. Next, the sample is transferred to a 100-millimeter centrifuge tube, and a second tube with an equal amount of water is placed opposite it in the centrifuge. The machine is run at 3500 rpm for three minutes. After centrifugation, the supernatant is poured off, and the tube is cleaned before adding sugar solution to mix with the pellet. After a second centrifugation, the process involves rinsing the solution, sieving the pellet through a small 500 mesh sieve, and storing the final sample in a 50 ml tube at 4°C until further analysis. Key steps emphasize contamination prevention, proper cleaning between samples, and precise handling measures to ensure consistent results.

Takeaways

  • 🌀 Begin by mixing the sample gently and placing it in a strainer.
  • 💧 Clean the strainer between samples to reduce contamination.
  • 🕳️ Pour the sample onto a 400 sieve at a 45-degree angle.
  • 🔄 Prepare the centrifuge with a balancing water tube opposite the sample.
  • ⚙️ Set the centrifuge to 3500 rpm for 3 minutes to centrifuge.
  • 🧪 Mix sugar solution with the pellet post-centrifugation.
  • 🚿 Rinse away sugar water through a 500 mesh sieve carefully.
  • 📦 Store the final sample in a tube at 4°C until analysis.
  • ✨ Emphasize cleaning of tools to avoid contamination.
  • 📏 Maintain precise handling for consistent results.

Timeline

  • 00:00:00 - 00:09:19

    Begin by gently mixing your sample and then pouring 100 CC into a small cup, which you then strain while blasting it with water. Ensure to clean the strainer between samples to avoid contamination. Wet your 400 sieve thoroughly before slowly pouring your sample, aiding the process by lightly patting and washing the sample to one side. Transfer the strain to a centrifuge tube, balancing it with another tube filled with water, and centrifuge them. Pour off the supernatant carefully and re-mix with a sugar solution, further centrifuge and transfer to a 500 mesh sieve for rinsing, then finally store in a 50ml tube at 4°C, ensuring cleanliness throughout.

Mind Map

Video Q&A

  • How do I start processing the sample?

    Begin by gently mixing the sample and then place 100 CC's of it into a strainer.

  • What should I do after placing the sample into the strainer?

    Blast the sample with a forceful stream of water to remove debris, and then dispose of the debris into a waste bin.

  • Why is it important to clean the strainer between samples?

    Cleaning the strainer between samples helps reduce contamination.

  • How should the sieve be prepared before pouring the sample?

    Thoroughly wet the 400 sieve and hold it at a 45-degree angle before slowly pouring the sample onto it.

  • How do I prepare the centrifuge for running a single sample?

    Fill a second centrifuge tube with water equal to that of the sample and place the two tubes opposite each other in the centrifuge.

  • What speed and duration should the centrifuge be set to?

    Set the centrifuge to run at 3500 rpm for 3 minutes.

  • What to do after centrifuging the sample with sugar solution?

    Slowly pour off the supernatant, keeping the small pellet at the bottom of the tube, then clean off the top of the tube of any debris.

  • How should the sugar solution be mixed with the sample pellet?

    Gently mix the sugar solution and the pellet together using a stirrer.

  • Where should the processed sample be stored?

    The sample can be stored in a 50 milliliter tube at 4 degrees Celsius until ready to be counted.

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  • 00:00:17
    begin by gently mixing the sample using
  • 00:00:26
    a small cup fill the cup with 100 CC's
  • 00:00:30
    of the sample and place the sample
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    directly into the strainer blast the
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    sample with forceful stream of water
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    remove the debris into a waste bin after
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    the sample has been blasted
  • 00:01:30
    it's important to clean the strainer
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    between each samples to reduce
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    contamination before pouring your sample
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    be sure to wet your 400 sieve thoroughly
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    while holding the sieve at a 45 degree
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    angle slowly pour the sample onto the
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    city gently Pat the sieve to remove
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    excess water while pouring
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    with a gentle stream of water slowly
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    wash the sample to one end of the sit
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    carefully transfer the sample from the
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    sieve into a 100 millimeter centrifuge
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    to thoroughly rinse the sieve and funnel
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    in between samples reduce contamination
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    when running a single sample fill a
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    second centrifuge tube with water equal
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    to that of the sample place the two
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    tubes opposite of each other in the
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    centrifuge latch the lid and set the
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    center feature run at 3500 rpm for 3
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    minutes allowing extra time for the
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    centrifuge to reach top speed
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    after centrifuging slowly pour off the
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    supernatant keeping the small pellet at
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    the bottom of the to be sure to clean
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    off the top of the tube of any debris
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    fill the centrifuge tube with sugar
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    solution stopping a half-inch
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    before the top of the tube using a stir
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    gently mix the sugar solution and pellet
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    together once the sugar solution and
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    pellet have been mixed return the tube
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    to the centrifuge for another 3 minutes
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    at 3500 rpm after centrifuging wet a
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    small 500 mesh sieve and pour the sample
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    onto the sieve
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    rinse away all the remaining sugar water
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    and move the sample to one side of the
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    500 measure sit
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    the sample can now be moved into a 50
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    milliliter - and stored at 4 degrees
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    Celsius until ready to be counted as
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    before rinse the sieve and funnel in
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    between samples
Tags
  • sample processing
  • centrifuge
  • sieve
  • contamination prevention
  • sample storage
  • sugar solution
  • centrifugation
  • straining method
  • precise handling
  • cleaning protocol