GRU Information Resource 1: Guide to how to cross peas.wmv

00:05:57
https://www.youtube.com/watch?v=Pq7-JGRmFBc

Resumo

TLDRThis video tutorial explains how to effectively cross-pollinate pea flowers. It outlines the necessary equipment and the proper steps for selecting and preparing flowers for pollination. The process includes sterilizing tweezers, selecting a bud-stage flower, carefully removing its petals to expose the stigma, and applying viable pollen from another flower using the anthers. Finally, the flowers are labeled for reference. The guide emphasizes the importance of timing for selecting flowers and how to manually transfer pollen without needing to bag the flowers post-process.

Conclusões

  • 🌸 Select flowers at the bud stage for best results.
  • 🔪 Sterilize tweezers between uses with ethanol.
  • 👋 Remove petals carefully to expose the stigma.
  • ⚗️ Use pollen from a male flower as a paintbrush.
  • 📝 Label the crossed flowers clearly for identification.

Linha do tempo

  • 00:00:00 - 00:05:57

    The demonstration begins with an introduction to the tools needed for crossing peas: sharp tweezers, a bottle of ethanol for sterilization, and labels. The speaker emphasizes the importance of selecting a flower at the right bud stage, as fully opened flowers are already pollinated and unusable. Once a suitable bud is found, the tweezers are used to carefully remove the petals to expose the anthers and the style, revealing the heart of the flower. This process is shown in detail to ensure successful pollination. The speaker then uses pollen from another flower to apply onto the style, which is visible and ready for cross-pollination. Instructions are repeated clearly to ensure understanding, including the identification of the female and male flowers and the precise method to apply pollen. Finally, the tagged flower is labeled to document the cross, concluding the demonstration of creating a crossed pea flower.

Mapa mental

Vídeo de perguntas e respostas

  • What equipment is needed for cross-pollination of pea flowers?

    Sharp tweezers, a bottle of ethanol for sterilization, and labels.

  • When is the right time to select flowers for cross-pollination?

    Select flowers at the bud stage before they fully open.

  • How do you prepare a flower for receiving pollen?

    Remove the petals to expose the anthers and style.

  • How is pollen applied to the recipient flower?

    Use the anthers of a male flower as a paintbrush to apply pollen to the stigma of the recipient flower.

  • Do you need to bag the flowers after cross-pollination?

    No, it's not necessary since they won't be visited by pollinators.

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Rolagem automática:
  • 00:00:01
    i'm going to demonstrate to you what you
  • 00:00:03
    need to do in order to cross peas
  • 00:00:07
    so the three pieces of equipment you
  • 00:00:09
    need
  • 00:00:11
    you need a sharp pair of tweezers
  • 00:00:15
    a small bottle of ethanol in which to
  • 00:00:17
    sterilize the tweezers in between going
  • 00:00:19
    between one plant and another
  • 00:00:21
    and some labels
  • 00:00:24
    so the first thing you need to do
  • 00:00:26
    is to select a flower at the right stage
  • 00:00:30
    so what we're looking for is a flower at
  • 00:00:32
    the bud stage before it's actually
  • 00:00:34
    opened by the time the flowers are fully
  • 00:00:37
    open like this these two
  • 00:00:40
    the flower is already pollinated and
  • 00:00:41
    that's no use
  • 00:00:43
    so in here we have earlier more immature
  • 00:00:46
    buds this one is at the bud stage and
  • 00:00:49
    should be a good candidate for crossing
  • 00:00:53
    so
  • 00:00:54
    what you do
  • 00:00:55
    is go in with tweezers and carefully
  • 00:00:57
    remove the petals
  • 00:01:00
    starting with the outer petals and then
  • 00:01:03
    going in and removing the keel
  • 00:01:09
    this clearly exposes the anthers and the
  • 00:01:13
    style
  • 00:01:17
    the heart of the flower
  • 00:01:20
    carefully take off the anthis
  • 00:01:28
    remove them all
  • 00:01:31
    there is the style ready to receive the
  • 00:01:33
    pollen
  • 00:01:36
    so that style is all ready to receive
  • 00:01:38
    pollen you don't have to wait
  • 00:01:40
    at all so
  • 00:01:42
    i'm going to come in with a flower from
  • 00:01:44
    another line here coloured flower line
  • 00:01:46
    which is more developed it will still
  • 00:01:49
    have
  • 00:01:50
    viable pollen in it and i'm going to
  • 00:01:52
    just
  • 00:01:54
    manually
  • 00:01:55
    hold back the petals
  • 00:01:58
    pull back the keel and that'll reveal
  • 00:02:02
    the stigma
  • 00:02:04
    with the pollen
  • 00:02:06
    on the end we can use this
  • 00:02:10
    as a paintbrush
  • 00:02:12
    to apply the pollen from this flower
  • 00:02:16
    onto the recipient
  • 00:02:18
    style
  • 00:02:28
    it's nice and visible you can see the
  • 00:02:30
    yellow pollen
  • 00:02:31
    so that is now ready
  • 00:02:33
    to be labelled up
  • 00:02:37
    so i'm going to go through the process
  • 00:02:39
    again
  • 00:02:40
    so
  • 00:02:41
    first of all you select your female
  • 00:02:43
    recipient flower
  • 00:02:45
    and you're looking for
  • 00:02:46
    a flower that's at the bud stage
  • 00:02:49
    here's a good one here
  • 00:02:56
    so you hold the flower in its correct
  • 00:02:58
    position and you
  • 00:03:00
    strip off
  • 00:03:01
    the petals going from the base of the
  • 00:03:04
    flower going either side of the keel
  • 00:03:08
    pulling the outer petals off first
  • 00:03:18
    and there's the keel with all the
  • 00:03:21
    anthers and the style inside you just go
  • 00:03:23
    in at the base of the keel
  • 00:03:26
    to thin
  • 00:03:28
    going at the base
  • 00:03:31
    you're
  • 00:03:32
    likely just to
  • 00:03:36
    go in without taking any of the other
  • 00:03:39
    structures out
  • 00:03:43
    taking a few of the answers there you
  • 00:03:45
    are you see the
  • 00:03:46
    remaining anthers and the stigmatic
  • 00:03:49
    surface in the
  • 00:03:51
    flower still there so
  • 00:03:54
    take off the remaining
  • 00:03:59
    and this
  • 00:04:04
    there you are
  • 00:04:06
    as the stigmatic surface ready to
  • 00:04:08
    receive pollen
  • 00:04:14
    so here's a
  • 00:04:15
    flower that is just about to open so
  • 00:04:18
    this flower is uh
  • 00:04:20
    the anthers will have dehisce inside so
  • 00:04:23
    it's no use to use as a female flower if
  • 00:04:26
    you go in pull back the outer
  • 00:04:30
    standard and wings
  • 00:04:33
    hold those in one hand
  • 00:04:37
    then firmly pull the keel from the base
  • 00:04:39
    pull it back
  • 00:04:40
    to expose
  • 00:04:43
    the style
  • 00:04:44
    which is full of pollen acts as a paint
  • 00:04:47
    brush
  • 00:04:48
    use that
  • 00:04:49
    to apply
  • 00:04:54
    the pollen large amount of pollen there
  • 00:04:59
    one flower because the male flower will
  • 00:05:02
    be enough to do several pollinations if
  • 00:05:05
    you get it at this stage
  • 00:05:07
    so the flower there is ready for tagging
  • 00:05:11
    i'm using these jewelers tags
  • 00:05:15
    the body is quite delicate so you want
  • 00:05:17
    to be able to sort of hold it and lasso
  • 00:05:20
    it carefully
  • 00:05:22
    and draw the string
  • 00:05:26
    around the base of that flower
  • 00:05:30
    clearly only one flower so that flower
  • 00:05:34
    has been crossed no need to bag it
  • 00:05:37
    it's not going to be visited you're not
  • 00:05:39
    going to get fallen
  • 00:05:41
    pulling on it now
  • 00:05:45
    the information of the cross is put onto
  • 00:05:47
    the label
  • 00:05:48
    and that's it
  • 00:05:50
    crossed pea flower
Etiquetas
  • cross-pollination
  • pea flowers
  • pollination techniques
  • flower anatomy
  • gardening
  • plant breeding
  • pollen application
  • bud stage
  • ethanol sterilization
  • tweezers