Logos Biosystems CELENA X Product Demo: How-to-use the high contents imaging and analysis system

00:09:01
https://www.youtube.com/watch?v=pnQe9cmE-sA

الملخص

TLDRIn this video, Bernard Lee introduces the Celina X software from Logos Biosystems, focusing on its functionality for setting up imaging experiments and performing data analysis. The software consists of two main components: Celina X Explorer, for designing imaging acquisitions, and Celina X Analyzer, for creating automatic image analysis pipelines. The video illustrates its user-friendly graphical interface, which enhances the ease of use compared to traditional software with top menus. Key features include customizable imaging settings, autofocus options, and the ability to design complex multi-dimensional time-lapse experiments. The tutorial walks through the steps of creating an experiment, adjusting settings for each imaging channel, specifying scan areas, and organizing image data for analysis, ultimately showcasing the software's capabilities in microscopy research.

الوجبات الجاهزة

  • 💻 Celina X offers user-friendly software for imaging experiments.
  • ⚙️ Two main components: Explorer for design; Analyzer for analysis.
  • 🖼️ Graphical interface simplifies access to settings.
  • 🔍 Supports image-based and laser-based autofocus.
  • 📊 Users can customize channel settings for experiments.
  • 🧪 Extensive templates for various vessel types available.
  • 🚦 Easy adjustment of focus and illumination settings.
  • 📐 Define specific scan areas for precision imaging.
  • ⏳ Time-lapse imaging capabilities included.
  • 📋 Review images by fields and time points post-acquisition.

الجدول الزمني

  • 00:00:00 - 00:09:01

    Bernard Lee introduces the Celina X software suite for imaging experiments, detailing its two main components: Celina X Explorer for designing imaging acquisitions and Celina X Analyzer for automated image analysis. The software features a user-friendly graphical interface without a traditional menu bar. It allows easy access to settings for channel selection, exposure, and focus adjustments, including image-based autofocus. Bernard demonstrates how to set up a sample experiment using fluorescent channels and a bright field channel, highlighting the use of predefined vessel templates and focusing techniques. He also illustrates how to define the scan area and configure a multi-dimensional imaging acquisition, including G stack and time-lapse imaging features, culminating in the initiation of the acquisition process.

الخريطة الذهنية

فيديو أسئلة وأجوبة

  • What are the two major software components of Celina X?

    The two major software components are Celina X Explorer and Celina X Analyzer.

  • How does the graphical interface of Celina X differ from other software?

    Celina X lacks a top menu bar and features a user-friendly graphical interface with easy access to settings.

  • What types of autofocus does Celina X support?

    It supports both image-based and laser-based autofocus.

  • What is the process to set up an imaging experiment?

    You can design an experiment by setting exposure times for each channel and choosing a scan area.

  • Can you add multiple imaging channels in an experiment?

    Yes, you can add various imaging channels like bright field and fluorescent channels.

  • How can you analyze acquired images?

    Acquired images can be reviewed per field and between time points after the acquisition.

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الترجمات
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التمرير التلقائي:
  • 00:00:02
    [Music]
  • 00:00:04
    hello I'm Bernard Lee and with the logos
  • 00:00:07
    Biosystems today we are going to have an
  • 00:00:11
    in-depth look into the software
  • 00:00:13
    functionality and how easy it is to set
  • 00:00:17
    up an imaging experiment and subsequent
  • 00:00:20
    data analysis Celina X comes with two
  • 00:00:24
    major software's one is Celina X
  • 00:00:28
    Explorer that lets you design from a
  • 00:00:32
    simple to a complex multi-dimensional
  • 00:00:34
    time-lapse imaging acquisition the
  • 00:00:38
    second components of the software is
  • 00:00:41
    called the Salina Excel analyzer that
  • 00:00:43
    gives you ability to design a customized
  • 00:00:48
    automatic image analysis and processing
  • 00:00:52
    pipeline for automated data output
  • 00:01:00
    unlike any other software what you may
  • 00:01:04
    notice is a lack or absence of a top
  • 00:01:07
    menu bar everything you need is laid out
  • 00:01:11
    in a very easy to understand graphical
  • 00:01:15
    interface in the software or components
  • 00:01:19
    such as land selections like channel and
  • 00:01:24
    related the exposure and illumination
  • 00:01:27
    settings are easily accessible by a
  • 00:01:29
    click of a button there is also a
  • 00:01:33
    control to manually adjust focus up and
  • 00:01:36
    down you can make use of image based
  • 00:01:40
    autofocus to find the best focal plane
  • 00:01:51
    yeah quickly found the best focal-plane
  • 00:01:55
    one of the biggest strengths of the
  • 00:01:58
    selena egg software is the use of a
  • 00:02:01
    graphical vessel template there are
  • 00:02:03
    templates available for every possible
  • 00:02:07
    vessel types from slide to different
  • 00:02:11
    multi-well plates they are listed by
  • 00:02:14
    product number and by manufacturer let's
  • 00:02:18
    start designing an actual experiment in
  • 00:02:20
    this sample acquisition I'm going to
  • 00:02:24
    design an acquisition with the two
  • 00:02:26
    fluorescent channels and one bright
  • 00:02:29
    field channel with an extensive scan
  • 00:02:33
    area and a one by three slide we are
  • 00:02:39
    going to use the 40x dry lens for our
  • 00:02:43
    scan the first thing we need to do is we
  • 00:02:48
    need to proceed to set an exporter time
  • 00:02:50
    for each channel as you can see the
  • 00:02:54
    channel settings including a specific
  • 00:02:58
    light illumination setting as well as
  • 00:03:01
    exposure time can be determined and set
  • 00:03:06
    into the experiment let's start with the
  • 00:03:09
    bright field I want to make the exports
  • 00:03:12
    a little bit brighter or maybe dimmer
  • 00:03:14
    yep and then I'm gonna add that to
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    experiment
  • 00:03:24
    so on the right hand side the
  • 00:03:27
    brightfield channel has been now entered
  • 00:03:29
    in the next channel that I'm going to do
  • 00:03:33
    is GRP and now I'm going to add this
  • 00:03:43
    channel but before I do that I'm going
  • 00:03:45
    to manually adjust the focal plane
  • 00:03:53
    [Music]
  • 00:03:55
    I'm going to add that to our experiment
  • 00:03:59
    Penner
  • 00:04:18
    [Music]
  • 00:04:22
    great now we're going to add sci-fi
  • 00:04:27
    channel maybe I will make it a little
  • 00:04:33
    bit more intense and then add a channel
  • 00:04:37
    to it
  • 00:04:50
    as I mentioned the Selena X comes with
  • 00:04:54
    forth image based autofocus and laser
  • 00:04:58
    based autofocus for our purpose we are
  • 00:05:02
    now going to show how we can set the
  • 00:05:05
    laser based autofocus what we're going
  • 00:05:07
    to do is we first going to switch back
  • 00:05:09
    to the break here and then test out the
  • 00:05:13
    laser based autofocus and we're going to
  • 00:05:20
    do this for every single field in the
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    image scan area let's test out the scan
  • 00:05:31
    [Music]
  • 00:05:34
    yep the focus is pretty well-defined so
  • 00:05:43
    now we set the laser autofocus as our
  • 00:05:48
    autofocus strategy the next thing we
  • 00:05:51
    need to do is we need to define the scan
  • 00:05:54
    area
  • 00:06:01
    you can see the current position on the
  • 00:06:04
    slide is marked with a little light blue
  • 00:06:08
    color when I said a arbitrary range and
  • 00:06:14
    maybe add few more yep right there okay
  • 00:06:22
    so we set about four by eight field of
  • 00:06:26
    view and then why don't we go ahead
  • 00:06:30
    might as well add a G stack of course we
  • 00:06:35
    can do simple two-dimensional scan with
  • 00:06:38
    the multi-channel but just for our
  • 00:06:40
    purpose of demonstrating that we can do
  • 00:06:43
    G stack I'm going to actually add a 5
  • 00:06:48
    micrometer
  • 00:06:49
    above and 5 micrometer below from the
  • 00:06:53
    current range and then set 3 steps in
  • 00:06:57
    between all four steps in between and
  • 00:07:00
    the Saltine automatically calculates
  • 00:07:02
    that it's going to do 2.5 micrometers
  • 00:07:06
    step size between the it's 4k plane and
  • 00:07:11
    of course we like to add maybe a
  • 00:07:17
    multiple intervals just to demonstrate
  • 00:07:20
    that we can do time-lapse imaging let's
  • 00:07:23
    do three and then and do as the
  • 00:07:26
    intervals are repeated as fast as
  • 00:07:30
    possible we're going to add that to our
  • 00:07:32
    strategy oh we need to select the vessel
  • 00:07:36
    and then add the cheese tag add the
  • 00:07:41
    time-lapse to our experiment now in the
  • 00:07:47
    experimental channel we're going to set
  • 00:07:49
    my field as our autofocus reference
  • 00:07:53
    channel and finally and most importantly
  • 00:07:58
    we need to assign a filename for this
  • 00:08:02
    experiment so let's do this
  • 00:08:05
    and I save it to D Drive and Cory test
  • 00:08:12
    one save now to start the acquisition
  • 00:08:19
    process we simply click run the software
  • 00:08:28
    is in total control until it completes
  • 00:08:31
    the entire position experiment once the
  • 00:08:34
    acquisition processes or complete you
  • 00:08:38
    can review your images from a field to a
  • 00:08:41
    field and between time points as well as
  • 00:08:44
    g4k planes
  • 00:08:46
    thank you for your attention for more
  • 00:08:49
    information please visit logos bio.com
  • 00:08:53
    thank you
  • 00:08:55
    [Music]
الوسوم
  • Celina X
  • Logos Biosystems
  • imaging software
  • data analysis
  • microscopy
  • autofocus
  • experiment design
  • image acquisition
  • multi-channel imaging
  • time-lapse imaging